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Test Code Amikacin Amikacin Level (Trough, Random, or Peak)

Additional Codes

LAB5029

Performing Laboratory

St. Louis Children's Hospital

Last Reviewed

November 2024

Lab Dept

Core Lab Chemistry

Specimen Requirements

Specimen Type: Blood

Container/Tube  
Preferred: Light green-top (Lithium heparin) Vacutainer
Acceptable: Plain, red-top Vacutainer or Lavender-top (EDTA) Vacutainer

Collection Volume: 2.0 mL (minimum 1.0 mL)
Patient Preparation: None
Sample Rejection: Mislabeled or unlabeled specimens.
Special Instructions: Trough Specimen: Collect immediately before next scheduled dose or no earlier than 1 hour prior to dose. (Trough level for first dose should be collected just prior to next dose).

Peak Specimen: Collect 30-60 minutes after dose.

Send to lab promptly.

General Information

EPIC order name:

Amikacin Level, Trough (EPIC Test # LAB5029)

Amikacin Level, Random (EPIC Test# LAB145)

Amikacin Level, Peak (EPIC Test# LAB5028)

Cerner order name:

Amikacin Tr

Amikacin Rnd

Amikacin Pk

CPT Codes: 80150
LOINC Value:

Amikacin Tr: 3321-7

Amikacin Rnd: 35669-1

Amikacin Pk: 3319-1

Synonyms:  

Interpretive

Therapeutic Range: ranges apply to all ages

Trough Level: <8.0 mcg/mL

Random Level: No established reference interval

Peak Level: 10.0-35.0 mcg/mL

Critical Values:

Random level: ≥75.0 mcg/mL

Trough level: >8.0 mcg/mL

Critical test results will be automatically called back initially and every 24 hours thereafter.
Limitations: NA
Methodology: Immunoassay (Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission).

Additional Information

For SLCH Laboratory use only

Lab Processing instructions: Centrifuge specimen to separate. If specimen is collected in a plain, red-top or Lavender-top (EDTA) tube; aliquot sample into a conical tube for testing (labeling with specimen type).
Processed Volume: 0.3 mL plasma/serum
Analyte Stability:

Ambient - unprocessed: 8 hours

PROCESSED:

Ambient: 8 hours
Refrigerated: 2 days
Frozen: 4 weeks
Notes: None

Logistics

Test Availability: Monday-Sunday
Cut-off time: None; performed as received
Turnaround Time: Same day; 2 hours from receipt in lab
Lab Testing Section: Chemistry