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Test Code LAB10102 Antinuclear Antibody (ANA) screen w/ reflex ENA and dsDNA

Additional Codes

LAB10102

Methodology

Immunofluorescent Antibody (IFA)

Testing Algorithm:

If ANA qualitative result is positive, then ANA  titer, ENA and dsDNA will be performed at an additional charge.

Literature References:

1. Peeples C. Fluorescent ANA’s and beyond! INOVA Diagnostics, Inc.; 2004.
2. Butcher B, Fritzler MJ, Gordon T, et al. Reference sera for antinuclear antibodies: II. Further definition of antibody specificities in international antinuclear antibody reference sera by immunofluorescence and western blotting. Am Coll Rheumatol 1997;40:413-18.

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Specimen Type: Serum

Container/Tube: Plain red top

Specimen Volume: Full tube

 

Specimen Transport Temperature

Ambient

Reference Values

ANA

Negative

Note: 1. Serum is assayed against HEp-2 (human epithelial) cultured cells.

2. A significantly positive ANA result is defined as positive nuclear fluorescence at a titer of ≥1:80.

3. Fifteen percent of normal people >65 years have significantly positive ANA results. Five percent or less of normal people ≤65 years have significantly positive ANA results.

 

ENA Screen

Negative

 

ds-DNA

<30 IUnits/mL (negative)

 

Day(s) Test Set Up

ANA TESTING

Monday through Friday

Cut-off time: 0800

Turnaround Time:
STAT: not available
Routine: results available the day of testing

ENA and dsDNA testing

Sunday - Saturday

Cut-off time: 0800

Turnaround Time:
STAT: not available
Routine: results available the day of testing

Test Classification and CPT Coding

86038-ANA screen

86039-ANA titer (if appropriate)

86235 - ENA screen (if appropriate)

86225-ds-DNA (if appropriate)

Additional Information

For BJH Laboratory Use Only

Alternate Tube:

SST Red top

Gold Top (serum SST)


Analyte Stability:

See analyte stabiity chart.

Laboratory Processing Instructions:

Test performed in BJH Core Lab. Processed by automated line and aliquot stored refrigerated for batch analysis. Completed specimens stored refrigerated for 5 days.