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Von Willebrand Factor (VWF) Multimers

Important Note

Send Stat to the Laboratory.  Specimen must be processed within 60 minutes of collection.

Additional Codes

LAB1111

Quest Test Code: 5168

Performing Laboratory

Quest Diagnostics Nichols Institute-Chantilly VA

Methodology

Electrophoresis

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Specimen Requirements

1 mL frozen platelet-poor plasma collected in a 3.2% sodium citrate (light blue-top) tube

 

Minimum Volume

0.5 mL

Collection Instructions

Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.

Note: Storage of whole blood at refrigerated temperatures prior to processing may lead to cryoprecipitate formation and falsely low Factor VIII and von Willebrand Factor studies

Specimen Transport Temperature

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen -70° C: 6 months

 

Reject Criteria

Hemolysis • Thawed plasma received room temperature or refrigerated

 

Day(s) Test Set Up

Set up: Mon-Thurs a.m.; Report available: 4-7 days

Reference Values

Normal

Test Classification and CPT Coding

85247