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HIT-Ab (PF4-H) Reflex Seratonin Release Assay (SRA)

Additional Codes

LAB29247

Performing Laboratory

MBMC Main Laboratory

Methodology

Latex enhanced immunoassay for the detection of anti-platelet factor 4/heparin (PF4-H)

Testing Algorithm

For positive HIT Antibody samples, a Serotonin Release Assay will reflex.  SRA will be sent to Versiti Labs (Blood Center of Wisconsin)

Specimen Requirements

Specimen is stabile for 8 hours at ambient temperature.

Specimen Type: Plasma

Container/Tube: 2.7-mL, light-blue top (3.2% sodium citrate)
Specimen Volume: Full tube

Specimen Minimum Volume: Fill to frosted minimal fill line on the tube.
Collection Instructions: It is imperative that the tube be completely filled to maintain the proper ratio of blood to anticoagulant.

 

Specimen Transport Temperature

SRA testing to Blood Center of Wisconsin:

Send aliquot specimen via FedEx overnight Priority -  Monday thru Friday refrigerated or with ice pack.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: within 4 hours

Blood Center of Wisconsin:  

Specimen must be received in the Laboratory by 1600 to ensure results by the next day (results are typically back in the afternoon by 5PM).

Reference Values

HIT antibody:  Negative

Test Classification and CPT Coding

86022

additional 86022, if appropriate

Stability

8 hours Ambiant temperature

Reflex SRA:  Separate plasma from cells asap and refrigerate to send to Blood Center of Wisconsin