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Immunofixation Electrophoresis, Urine

Important Note

24 Hour Urine is Preferred. Random Urine is accepted at Pathologist’s discretion and requires a minimum of 30 mL from first morning collection.

Additional Codes

LAB6532

Performing Laboratory

MBMC Main Laboratory

Methodology

Gel Electrophoresis/Immunoprecipitation

Note: This test is automatically performed when Protein Electrophoresis, 24 Hour, Urine is ordered.

Literature Reference:

Whicher JT, Hawkins L, Higginson J. Clinical applications of immunofixation: a more sensitive technique for the detection of Bence-Jones protein. J Clin Pathol 988;33:779-80.

Specimen Requirements

Submit only 1 of the following specimens:

 

Preferred

Specimen Type: Urine, 24-hour

Container/Tube: Plastic urine container

Specimen Volume: Entire 24-hour collection; Facilities outside of MBC should submit at least 30 mL of urine of the 24 hour collection

Collection Instructions: No preservative. Refrigerate specimen during collection.

Additional Information:

1. Starting and ending times of collection are required.

2. Follow instructions in Urine Collection in Special Instructions.

 

Specimen Type: Urine, random

Container/Tube: Urine Complete Cup Kit

Specimen Volume: 30 mL from a first-morning collection.

Collection Instructions: No additive.

Additional Information: See Urine Collection in Special Instructions for additional information.

Specimen Transport Temperature

Ambient

Day(s) Test Set Up

Monday through Friday

Cut-off time: 0800
Turnaround Time:
STAT: not available
Routine: 2 working days

Reference Values

No monoclonal protein detected.

Literature Reference:

Alper CA, Johnson AM. Immunofixation electrophoresis: a technique for the study of protein polymorphism. Vox Sang 1969;17:445-52.

 

 

Test Classification and CPT Coding

CPT Code:
81050-Volume measurement for timed collection

86335-Immunofixation