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Test Code LAB6998 Enterovirus RNA Detection by PCR, CSF

Additional Codes

LAB6998

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Polymerase Chain Reaction (PCR) performed on the Diasorin Liaison MDX

Specimen Requirements

Acceptable Specimens:

 

Cerebrospinal fluid (CSF): > 1 mL in a leakproof screw-capped, sterile container

 

Collection Procedure:

1. Aseptically collect ≥1 mL of CSF.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and transport promptly to laboratory.
4. If transport is delayed, refrigerate specimen.
5. If transport is delayed greater than 72 hours, freeze specimen.


Notes:

1. Specimen source is required.
2. Laboratory medicine resident review and approval is required for CSF specimen requests on BJH patients.
 

Day(s) Test Set Up

Monday through Sunday


Turnaround Time:


STAT: not available
Routine: 12 hours
 

Reference Values

Not Detected

Test Classification and CPT Coding

CPT Code:

87498
 

Additional Information

For BJH Laboratory Use Only
Minimum Volume:
Cerebrospinal (CSF): 0.4 mL

 

Laboratory Processing Instructions

Test performed in BJH Molecular Infectious Disease

 

Limitations

  1. The detection of viral nucleic acid is dependent upon proper sample collection, transport, handling and storage. Failure to observe proper procedures in any one of these steps can lead to incorrect results.
  2. False-negative results may occur if the viral material is present at a level that is below the analytical sensitivity of the assay or if the bacterium has genomic mutations, insertions, deletions, or rearrangements or if performed very early in the course of illness.


 

Specimen Transport Temperature

CSF – Ambient/Refrigerate/Frozen/OK
If transport is delayed, refrigerate specimen.
If transport is delayed greater than 72 hours, freeze specimen.