Respiratory Pathogen Multiplex PCR
Additional Codes
LAB7307
Performing Laboratory
MBMC Microbiology Laboratory
Methodology
Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Specimen Requirements
Specimen Type: NP Swab, Bronchial Alveolar lavage fluid, Bronchial wash, sputum, or tracheal aspirate.
Container: SUBMIT ONLY 1 OF THE FOLLOWING SPECIMENS based on clinical presentation of symptoms
Collection:
Nasopharyngeal Swab
- Obtain a vial of universal/viral transport medium (UTM) with collection swab from Missouri Baptist Medical Center Microbiology lab.
- Collect specimen by inserting swab into nares, and into nasopharyngeal area.
- Rotate swab to collect epithelial cells from nasopharyngeal area. Hold swab in place for 10-15 seconds to increase specimen collection.
- Place the swab in UTM vial, break off shaft of swab and discard excess piece of shaft. To prevent leaking, make sure the swabs do not interfere with tightening cap on vial.
- Label transport container with 2 patient identifiers and date and time of collection.
- Tightly cap the tube, place in a transport bag, and seal.
- If transport time will exceed 4 hours, specimen should be transported with cold/ice pack
Bronchial Alveolar Lavage (BAL), Bronch Wash, Sputum, or Tracheal Aspirate*
*These specimen types will go to BJH for testing.
- Aseptically collect 1.0 mL of specimen.
- Place specimen in a screw-capped, sterile container.
- Label transport container with 2 patient identifiers and with date and time of collection.
- Maintain sterility and forward promptly.
- If transport time will exceed 4 hours, specimen should be transported with cold/ice pack.
Specimen Transport Temperature
If specimen will be in transit >4 hours, it should be transported with a cold pack. Specimen may be held at 2-8° for up to 3 days or at <15° for up to 30 days.
Day(s) Test Set Up
Monday-Sunday
TAT: 2 hours from receipt in Laboratory
Test Classification and CPT Coding
0202U - RESP PATHOGEN 22 TARGETS
Test Components
This test is capable of detecting the following respiratory pathogens:
Bordetella pertussis
Bordetella parapertussis
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Adenovirus
Coronavirus HKU1
Coronavirus NL63
Coronavirus 229E
Coronavirus OC43
Influenza A
Influenza A subtype H1
Influenza A subtype H3
Influenza A subtype 2009 H1N1
Influenza B
Metapneumovirus
Parainfluenza 1
Parainfluenza 2
Parainfluenza 3
Parainfluenza 4
RSV
Rhinovirus/Enterovirus
SARS-CoV-2