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Respiratory Pathogen Multiplex PCR

Infectious

Additional Codes

LAB7307

Performing Laboratory

MBMC Microbiology Laboratory

Methodology

Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Specimen Requirements

Specimen Type: NP Swab, Bronchial Alveolar lavage fluid, Bronchial wash, sputum, or tracheal aspirate.

 

Container: SUBMIT ONLY 1 OF THE FOLLOWING SPECIMENS based on clinical presentation of symptoms

 

Collection:

 

Nasopharyngeal Swab

  1. Obtain a vial of universal/viral transport medium (UTM) with collection swab from Missouri Baptist Medical Center Microbiology lab.
  2. Collect specimen by inserting swab into nares, and into nasopharyngeal area.
  3. Rotate swab to collect epithelial cells from nasopharyngeal area. Hold swab in place for 10-15 seconds to increase specimen collection. 
  4. Place the swab in UTM vial, break off shaft of swab and discard excess piece of shaft. To prevent leaking, make sure the swabs do not interfere with tightening cap on vial.
  5. Label transport container with 2 patient identifiers and date and time of collection.
  6. Tightly cap the tube, place in a transport bag, and seal.
  7. If transport time will exceed 4 hours, specimen should be transported with cold/ice pack

  Bronchial Alveolar Lavage (BAL), Bronch Wash, Sputum, or Tracheal Aspirate*

*These specimen types will go to BJH for testing.

  1. Aseptically collect 1.0 mL of specimen.
  2. Place specimen in a screw-capped, sterile container.
  3. Label transport container with 2 patient identifiers and with date and time of collection.
  4. Maintain sterility and forward promptly.
  5. If transport time will exceed 4 hours, specimen should be transported with cold/ice pack.   

Specimen Transport Temperature

If specimen will be in transit >4 hours, it should be transported with a cold pack.   Specimen may be held at 2-8° for up to 3 days or at <15° for up to 30 days.

Day(s) Test Set Up

Monday-Sunday   

TAT: 2 hours from receipt in Laboratory

Test Classification and CPT Coding

0202U - RESP PATHOGEN 22 TARGETS

Test Components

This test is capable of detecting the following respiratory pathogens:

Bordetella pertussis

Bordetella parapertussis

Chlamydophila pneumoniae

Mycoplasma pneumoniae

Adenovirus

Coronavirus HKU1

Coronavirus NL63

Coronavirus 229E

Coronavirus OC43

Influenza A

Influenza A subtype H1

Influenza A subtype H3

Influenza A subtype 2009 H1N1

Influenza B

Metapneumovirus

Parainfluenza 1

Parainfluenza 2

Parainfluenza 3

Parainfluenza 4

RSV

Rhinovirus/Enterovirus

SARS-CoV-2